Cytoplasmic Control of Sense-Antisense mRNA Pairs

Flore Sinturel (1) , Albertas Navickas (1) , Maxime Wery (2) , Marc Descrimes (2, 3) , Antonin Morillon (2) , Claire Torchet (1) , Lionel Benard (1)

1 LBMCE - Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes
2 DIG CANCER - Dynamique de l'information génétique : bases fondamentales et cancer
3 Dynamics of genetic information : fundamental basis and cancer

Transcriptome analyses have revealed that convergent gene transcription can produce many 3′-overlapping mRNAs in diverse organisms. Few studies have examined the fate of 3′-complementary mRNAs in double-stranded RNA-dependent nuclear phenomena, and nothing is known about the cytoplasmic destiny of 3′-overlapping messengers or their impact on gene expression. Here, we demonstrate that the complementary tails of 3′-overlapping mRNAs can interact in the cytoplasm and promote post-transcriptional regulatory events including no-go decay (NGD) in Saccharomyces cerevisiae. Genome-wide experiments confirm that these messenger-interacting mRNAs (mimRNAs) form RNA duplexes in wild-type cells and thus have potential roles in modulating the mRNA levels of their convergent gene pattern under different growth conditions. We show that the post-transcriptional fate of hundreds of mimRNAs is controlled by Xrn1, revealing the extent to which this conserved 5′-3′ cytoplasmic exoribonuclease plays an unexpected but key role in the post-transcriptional control of convergent gene expression.

Domaines: Biochimie, Biologie Moléculaire; Biologie cellulaire

Flore Sinturel, Albertas Navickas, Maxime Wery, Marc Descrimes, Antonin Morillon, et al.. Cytoplasmic Control of Sense-Antisense mRNA Pairs. Cell Reports, 2015, 12 (11), pp.1853-1864. ⟨10.1016/j.celrep.2015.08.016⟩. ⟨hal-01216461⟩