Evidence for new C-terminally truncated variants of - and -tubulins - Archive ouverte HAL Access content directly
Journal Articles Molecular Biology of the Cell Year : 2016

Evidence for new C-terminally truncated variants of - and -tubulins

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Abstract

Cellular α-tubulin can bear various carboxy-terminal sequences: full-length tubulin arising from gene neosynthesis is tyrosinated, and two truncated variants, corresponding to detyrosinated and Δ2 α‑tubulin, result from the sequential cleavage of one or two C-terminal residues, respectively. Here, by using a novel antibody named 3EG that is highly specific to the -EEEG C-terminal sequence, we demonstrate the occurrence in neuronal tissues of a new αΔ3‑tubulin variant corresponding to α1A/B‑tubulin deleted of its last three residues (EEY). αΔ3‑tubulin has a specific distribution pattern: its quantity in the brain is similar to that of αΔ2-tubulin around birth but is much lower in adult tissue. This truncated α1A/B-tubulin variant can be generated from αΔ2-tubulin by the deglutamylases CCP1, CCP4, CCP5, and CCP6 but not by CCP2 and CCP3. Moreover, using 3EG antibody, we identify a C‑terminally truncated β-tubulin form with the same -EEEG C-terminal sequence. Using mass spectrometry, we demonstrate that β2A/B-tubulin is modified by truncation of the four C-terminal residues (EDEA). We show that this newly identified βΔ4-tubulin is ubiquitously present in cells and tissues and that its level is constant throughout the cell cycle. These new C-terminally truncated α- and β-tubulin variants, both ending with -EEEG sequence, are expected to regulate microtubule physiology. Of interest, the αΔ3-tubulin seems to be related to dynamic microtubules, resembling tyrosinated-tubulin rather than the other truncated variants, and may have critical function(s) in neuronal development.
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hal-01405743 , version 1 (08-06-2021)

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Attribution - NonCommercial - ShareAlike - CC BY 4.0

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Chrystelle Aillaud, Christophe Bosc, Yasmina Saoudi, Eric Denarier, Leticia Peris, et al.. Evidence for new C-terminally truncated variants of - and -tubulins. Molecular Biology of the Cell, 2016, 27 (4), pp.640 - 653. ⟨10.1091/mbc.E15-03-0137⟩. ⟨hal-01405743⟩
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