20-nm-sized mesoporous silica nanoparticles with porphyrin photosensitizers for in vitro photodynamic therapy

Chiara Mauriello Jimenez (1) , Yolanda Galàn Rubio (1) , Valentin Saunier (1) , David Warther (1) , Vanja Stojanovic (2) , Laurence Raehm (1) , Céline Frochot (3) , Philippe Arnoux (3) , Marcel Garcia (2) , Alain Morère (2) , Nadir Bettache (2) , Marie Maynadier (2) , Philippe Maillard (4) , Magali Gary-Bobo (2) , Jean-Olivier Durand (1)

1 ICGM - Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier
2 IBMM - Institut des Biomolécules Max Mousseron [Pôle Chimie Balard]
3 LRGP - Laboratoire Réactions et Génie des Procédés
4 Institut Curie [Paris]

We report the synthesis of 20-nm-sized mesoporous silica nanoparticles encapsulating three different porphyrin photosensitizers (PS). Synthesized by the sol–gel method, in the presence of cetyltrimethylammonium bromide surfactant and tetraorthosilicate as silica source, we developed those nanoparticles with the aim of treating cancer cells using photodynamic therapy. The colloidal stability, due to the PEG-silane chain grafted on the surface, was demonstrated at 37 C in cell culture medium. Then, nanoparticles were functionalized by silylated squarate mannose and used in MCF-7 breast cancer cells for one-photon therapy. Promising results were obtained after 5 h of incubation.

Mots clés: Functionalization; Mesoporous; Mesoporous; Silica nanoparticles; silica; Silica nanoparticles; nanoparticles; PDT; PDT; Porphyrin; functionalization

Domaines: Matériaux; Chimie thérapeutique; Biologie cellulaire

Chiara Mauriello Jimenez, Yolanda Galàn Rubio, Valentin Saunier, David Warther, Vanja Stojanovic, et al.. 20-nm-sized mesoporous silica nanoparticles with porphyrin photosensitizers for in vitro photodynamic therapy. Journal of Sol-Gel Science and Technology, 2016, 79 (3), pp.447-456. ⟨10.1007/s10971-016-3991-6⟩. ⟨hal-01569372⟩