A high-risk retinoblastoma subtype with stemness features, dedifferentiated cone states and neuronal/ganglion cell gene expression

Jing Liu (1, 2) , Daniela Ottaviani (3, 1) , Meriem Sefta (1) , Céline Desbrousses (1) , Elodie Chapeaublanc (1) , Rosario Aschero (3) , Nanor Sirab (1) , Fabiana Lubieniecki (3) , Gabriela Lamas (3) , Laurie Tonon (4, 5) , Catherine Dehainault (6, 7) , Clément Hua (1) , Paul Fréneaux (6) , Sacha Reichman (8) , Narjesse Karboul (1) , Anne Biton (1, 9, 10, 11) , Liliana Mirabal-Ortega (12) , Magalie Larcher (12) , Céline Brulard (1, 13, 14) , Sandrine Arrufat (6) , André Nicolas (6) , Nabila Elarouci (2) , Tatiana Popova (15) , Fariba Némati (16) , Didier Decaudin (16) , David Gentien (16) , Sylvain Baulande (17) , Odette Mariani (6) , Florent Dufour (1) , Sylvain Guibert (18) , Céline Vallot (18) , Livia Lumbroso-Le Rouic (17) , Alexandre Matet (17) , Laurence Desjardins (17) , Guillem Pascual-Pasto (19, 20) , Mariona Suñol (19, 20) , Jaume Catala-Mora (19, 20) , Genoveva Correa Llano (19, 20) , Jérôme Couturier (6) , Emmanuel Barillot (9) , Paula Schaiquevich (3, 21) , Marion Gauthier-Villars (6, 7, 15) , Dominique Stoppa-Lyonnet (6, 7) , Lisa Golmard (6, 15) , Claude Houdayer (6, 15, 22) , Hervé Brisse (23) , Isabelle Bernard-Pierrot (1) , Eric Letouzé (24, 25) , Alain Viari (4, 5) , Simon Saule (12) , Xavier Sastre-Garau (6, 26) , François Doz (17) , Angel Carcaboso (19, 20) , Nathalie Cassoux (7) , Celio Pouponnot (12) , Olivier Goureau (8) , Guillermo Chantada (19, 3, 20, 21) , Aurélien de Reyniès (2) , Isabelle Aerts (1) , François Radvanyi (1)

1 Biologie Cellulaire et Cancer
2 CIT - (le programme) Cartes d'identité des tumeurs
3 Hospital Nacional de Pediatría J.P. Garrahan
4 Synergie Lyon Cancer-Platform of Bioinformatics-Gilles Thomas
5 Centre Léon Bérard [Lyon]
6 Département de Biologie des Tumeurs
7 Institut Curie [Paris]
8 Institut de la Vision
9 Cancer et génome: Bioinformatique, biostatistiques et épidémiologie d'un système complexe
10 Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB
11 C3BI - Centre de Bioinformatique, Biostatistique et Biologie Intégrative
12 Signalisation, radiobiologie et cancer
13 iBrain - Inserm U1253 - UNIV Tours - Imagerie et cerveau
14 Hôpital Bretonneau
15 U830 - Unité de génétique et biologie des cancers
16 Département de Recherche Translationnelle
17 Centre de recherche de l'Institut Curie [Paris]
18 GeCo - Genomics Consulting
19 Institut de Recerca Pediàtrica Hospital Sant Joan de Déu [Barcelona, Spain]
20 Hospital Sant Joan de Déu [Barcelona]
21 CONICET - Consejo Nacional de Investigaciones Científicas y Técnicas [Buenos Aires]
22 CHU Rouen
23 Département d'Imagerie Médicale [Institut Curie]
24 FunGeST - Génomique fonctionnelle des tumeurs solides = Functional Genomics of Solid Tumors [CRC]
25 CRC (UMR_S_1138 / U1138) - Centre de Recherche des Cordeliers
26 CHI Créteil

Retinoblastoma is the most frequent intraocular malignancy in children, originating from a maturing cone precursor in the developing retina. Little is known on the molecular basis underlying the biological and clinical behavior of this cancer. Here, using multi-omics data, we demonstrate the existence of two retinoblastoma subtypes. Subtype 1, of earlier onset, includes most of the heritable forms. It harbors few genetic alterations other than the initiating RB1 inactivation and corresponds to differentiated tumors expressing mature cone markers. By contrast, subtype 2 tumors harbor frequent recurrent genetic alterations including MYCN -amplification. They express markers of less differentiated cone together with neuronal/ganglion cell markers with marked inter- and intra-tumor heterogeneity. The cone dedifferentiation in subtype 2 is associated with stemness features including low immune and interferon response, E2F and MYC/MYCN activation and a higher propensity for metastasis. The recognition of these two subtypes, one maintaining a cone-differentiated state, and the other, more aggressive, associated with cone dedifferentiation and expression of neuronal markers, opens up important biological and clinical perspectives for retinoblastomas.

Domains: Life Sciences [q-bio]; Cancer

Jing Liu, Daniela Ottaviani, Meriem Sefta, Céline Desbrousses, Elodie Chapeaublanc, et al.. A high-risk retinoblastoma subtype with stemness features, dedifferentiated cone states and neuronal/ganglion cell gene expression. Nature Communications, 2021, 12 (1), pp.1-20. ⟨10.1038/s41467-021-25792-0⟩. ⟨hal-03374490⟩