The GTPase Rab8 differentially controls the long- and short-range activity of the Hedgehog morphogen gradient by regulating Hedgehog apico-basal distribution - Archive ouverte HAL Access content directly
Journal Articles Development (Cambridge, England) Year : 2021

The GTPase Rab8 differentially controls the long- and short-range activity of the Hedgehog morphogen gradient by regulating Hedgehog apico-basal distribution

, (1) , (2) , , , (1) , (3) , (1)
1
2
3

Abstract

ABSTRACT The Hedgehog (Hh) morphogen gradient is required for patterning during metazoan development, yet the mechanisms involved in Hh apical and basolateral release and how this influences short- and long-range target induction are poorly understood. We found that depletion of the GTPase Rab8 in Hh-producing cells induces an imbalance between the level of apically and laterally released Hh. This leads to non-cell-autonomous differential effects on the expression of Hh target genes, namely an increase in its short-range targets and a concomitant decrease in long-range targets. We further found that Rab8 regulates the endocytosis and apico-basal distribution of Ihog, a transmembrane protein known to bind to Hh and to be crucial for establishment of the Hh gradient. Our data provide new insights into morphogen gradient formation, whereby morphogen activity is functionally distributed between apically and basolaterally secreted pools.
Fichier principal
Vignette du fichier
dev191791.pdf (21.08 Mo) Télécharger le fichier
Origin : Explicit agreement for this submission

Dates and versions

hal-03414597 , version 1 (08-11-2021)

Identifiers

Cite

Tanvi Gore, Tamás Matusek, Gisela d'Angelo, Cécile Giordano, Thomas Tognacci, et al.. The GTPase Rab8 differentially controls the long- and short-range activity of the Hedgehog morphogen gradient by regulating Hedgehog apico-basal distribution. Development (Cambridge, England), 2021, 148 (5), ⟨10.1242/dev.191791⟩. ⟨hal-03414597⟩
58 View
2 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More