The human TREX-2 complex is stably associated with the nuclear pore basket - Archive ouverte HAL Access content directly
Journal Articles Journal of Cell Science Year : 2013

The human TREX-2 complex is stably associated with the nuclear pore basket

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Abstract

In eukaryotes mRNA export involves many evolutionarily conserved factors that carry the nascent transcript to the nuclear pore complex (NPC). The THO/TREX complex couples transcription to mRNA export and recruits the mRNA export receptor NXF1 for the transport of mRNP particles to the NPC. The transcription and export complex 2 (TREX-2) was suggested to interact with NXF1 and to shuttle between transcription sites and the NPC. Here, we characterize the dynamics of human TREX-2 and show that it stably associates with the NPC basket. Moreover, the association of TREX-2 with the NPC requires the basket nucleoporins NUP153 and TPR, but is independent of transcription. Differential profiles of mRNA nuclear accumulation reveal that TREX-2 functions similarly to basket nucleoporins, but differently from NXF1. Thus, our results show that TREX-2 is an NPC-associated complex in mammalian cells and suggest that it is involved in putative NPC basket-related functions.

Dates and versions

hal-03464588 , version 1 (03-12-2021)

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David Umlauf, Jacques Bonnet, François Waharte, Marjorie Fournier, Matthieu Stierle, et al.. The human TREX-2 complex is stably associated with the nuclear pore basket. Journal of Cell Science, 2013, ⟨10.1242/jcs.118000⟩. ⟨hal-03464588⟩
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