Activity-driven extracellular volume expansion drives vertebrate axis elongation - Institut Curie Accéder directement au contenu
Pré-Publication, Document De Travail Année : 2022

Activity-driven extracellular volume expansion drives vertebrate axis elongation

Résumé

Abstract The vertebrate bauplan is primarily established via the formation of embryonic tissues in a head-to-tail progression. The biomechanics of this elongation, which requires the presomitic mesoderm (PSM), remains poorly understood. Here, we find that avian PSM explants can elongate autonomously when physically confined in vitro , producing a pushing force that can largely account for the posterior elongation of the embryo. Tissue elongation results from volumetric expansion that is driven by cellular activity and accompanied by inhomogeneous increase of the extracellular fraction along the AP axis. We show that FGF signaling promotes glycolysis-dependent production of Hyaluronic Acid (HA), which is required for expansion of the posterior PSM. Our findings link body axis elongation to tissue expansion through the metabolic control of extracellular matrix production downstream of FGF signaling. One-Sentence Summary Active tissue expansion propels body elongation independent of cell proliferation-driven growth

Dates et versions

hal-03872683 , version 1 (25-11-2022)

Identifiants

Citer

Arthur Michaut, Alessandro Mongera, Anupam Gupta, Mattia Serra, Pietro Rigoni, et al.. Activity-driven extracellular volume expansion drives vertebrate axis elongation. 2022. ⟨hal-03872683⟩
14 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More