Polymorphic microsatellite loci in the widespread amphidromous goby Sicyopterus lagocephalus and cross-genus amplification among Sicydiinae

Thierry B. Hoareau (1) , Federica Barbisan (2) , Sophie Dubois (3) , Lorenzo Zane (2) , Patrick Berrebi (3, 4)

1 ECOMAR - Laboratoire d'Ecologie Marine
2 Unipd - Università degli Studi di Padova = University of Padua
3 UMR ISEM - Institut des Sciences de l'Evolution de Montpellier
4 ECOLAG - Ecosystèmes lagunaires : organisation biologique et fonctionnement

Microsatellite DNA markers were isolated in an amphidromous goby (Sicyopterus lagocephalus) from a partial genomic library enriched for AC repeats. Eight microsatellites were highly polymorphic with six to 33 alleles per locus and expected heterozygosities ranging from 0.53 to 0.97. Cross-species amplifications were performed within the sub-family Sicydiinae by genotyping individuals from two species of the genus Cotylopus. Some of these loci were successfully amplified and showed polymorphism in the second genus.

Mots clés: amphidromous gobiids; cross-genus amplification; Genetic markers; microsatellite; Sicydiins; Wahlund effect

Thierry B. Hoareau, Federica Barbisan, Sophie Dubois, Lorenzo Zane, Patrick Berrebi. Polymorphic microsatellite loci in the widespread amphidromous goby Sicyopterus lagocephalus and cross-genus amplification among Sicydiinae. Molecular Ecology Resources, 2009, 9 (2), pp.607 - 609. ⟨10.1111/j.1755-0998.2008.02461.x⟩. ⟨halsde-00366231⟩