The Rice Resistance Protein Pair RGA4/RGA5 Recognizes the Magnaporthe oryzae Effectors AVR-Pia and AVR1-CO39 by Direct Binding - Laboratoire des Matériaux Avancés Accéder directement au contenu
Article Dans Une Revue The Plant cell Année : 2013

The Rice Resistance Protein Pair RGA4/RGA5 Recognizes the Magnaporthe oryzae Effectors AVR-Pia and AVR1-CO39 by Direct Binding

S. Cesari
  • Fonction : Auteur
G. Thilliez
  • Fonction : Auteur
C. Ribot
  • Fonction : Auteur
V. Chalvon
  • Fonction : Auteur
C. Michel
S. Rivas
  • Fonction : Auteur
L. Alaux
  • Fonction : Auteur
H. Kanzaki
  • Fonction : Auteur
Y. Okuyama
  • Fonction : Auteur
J.-B. Morel
E. Fournier
T. Kroj

Résumé

Resistance (R) proteins recognize pathogen avirulence (Avr) proteins by direct or indirect binding and are multidomain proteins generally carrying a nucleotide binding (NB) and a leucine-rich repeat (LRR) domain. Two NB-LRR protein-coding genes from rice (Oryza sativa), RGA4 and RGA5, were found to be required for the recognition of the Magnaporthe oryzae effector AVR1-CO39. RGA4 and RGA5 also mediate recognition of the unrelated M. oryzae effector AVR-Pia, indicating that the corresponding R proteins possess dual recognition specificity. For RGA5, two alternative transcripts, RGA5-A and RGA5-B, were identified. Genetic analysis showed that only RGA5-A confers resistance, while RGA5-B is inactive. Yeast two-hybrid, coimmunoprecipitation, and fluorescence resonance energy transfer-fluorescence lifetime imaging experiments revealed direct binding of AVR-Pia and AVR1-CO39 to RGA5-A, providing evidence for the recognition of multiple Avr proteins by direct binding to a single R protein. Direct binding seems to be required for resistance as an inactive AVR-Pia allele did not bind RGA5-A. A small Avr interaction domain with homology to the Avr recognition domain in the rice R protein Pik-1 was identified in the C terminus of RGA5-A. This reveals a mode of Avr protein recognition through direct binding to a novel, non-LRR interaction domain.

Dates et versions

hal-01759652 , version 1 (05-04-2018)

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Citer

S. Cesari, G. Thilliez, C. Ribot, V. Chalvon, C. Michel, et al.. The Rice Resistance Protein Pair RGA4/RGA5 Recognizes the Magnaporthe oryzae Effectors AVR-Pia and AVR1-CO39 by Direct Binding. The Plant cell, 2013, 25 (4), pp.1463 - 1481. ⟨10.1105/tpc.112.107201⟩. ⟨hal-01759652⟩
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