An approach of a study of the interaction between collagen and sulphur dioxide by using ESI and MALDI-TOFMS

K. Dif (1) , C. Pepe (2) , J. Peduzzi (3) , B. Lavedrine (1) , C. Chahine (1)

1 CRCDG - Centre de recherches sur la conservation des documents graphiques
2 LADIR - Laboratoire de Dynamique Interactions et Réactivité
3 Laboratoire de chimie et biochimie des substances naturelles

In order to further understand the interaction between pollutants and collagen, a study with two plain proteins, bovine serum albumin (BSA) and lysozyme, both exposed to SO2 has been carried out. X-ray analysis of the samples using a scanning microscope spectrometer (SEM) was carried out. Samples were analysed by mass spectrometry using an electrospray source coupled to a time of flight analyser (ESI-TOFMS) and by Fourier transform infrared spectroscopy (FT-IR). A chemical cleavage using cyanogen bromide (CNBr) was carried out on BSA exposed to SO2, the CNBr cleavage fragments were analysed by matrix-assisted laser desorption/ionization source coupling to a TOF analyser (MALDI-TOFMS). All these results allow us to determine a hypothesis for the mechanism of the reaction between SO2 and BSA.

Mots clés: collagen; bovine serum albumine; sulphur dioxyde; pollution; mass spectrometry

Domaines: Biochimie [q-bio.BM]

K. Dif, C. Pepe, J. Peduzzi, B. Lavedrine, C. Chahine. An approach of a study of the interaction between collagen and sulphur dioxide by using ESI and MALDI-TOFMS. Journal of Cultural Heritage, 2002, 3, pp.317-323. ⟨10.1016/S1296-2074(02)01241-4⟩. ⟨hal-00013200⟩