Screening the active constituents of Chinese medicinal herbs as potent inhibitors of Cdc25 tyrosine phosphatase, an activator of the mitosis-inducing p34cdc2 kinase - Université Pierre et Marie Curie Accéder directement au contenu
Article Dans Une Revue Journal of Zhejiang University. Science Année : 2005

Screening the active constituents of Chinese medicinal herbs as potent inhibitors of Cdc25 tyrosine phosphatase, an activator of the mitosis-inducing p34cdc2 kinase

H. Yang
  • Fonction : Auteur
S. Zheng
  • Fonction : Auteur
Li S. M.
  • Fonction : Auteur
S. Leclerc
  • Fonction : Auteur
Yu Lin-Lin
  • Fonction : Auteur
Cheng J. Q.
  • Fonction : Auteur
Zhang S. Z.
  • Fonction : Auteur

Résumé

OBJECTIVE: To screen and evaluate the active constituents of Chinese medicinal herbs as potent inhibitors of Cdc25 phosphatase. METHODS: The affinity chromatography purified glutashione-S-transferase/Cdc25A phosphatase fusion protein and Cdc2/cyclin B from the extracts of starfish M phase oocytes are used as the cell cycle-specific targets for screening the antimitotic constituents. We tested 9 extracts isolated from the Chinese medicinal herbs and vegetables including the agents currently used in cancer treatment by measuring the inhibition of Cdc25A phosphatase and Cdc2 kinase activity. The antitumor activity of the extracts was also evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and flow cytometry. RESULTS: Cdc25A inhibitory activity and antitumor activity are detected in the extracts isolated from three Chinese medicinal herbs Agrimona pilosa; Herba solani lyrati; Galla chinesis. CONCLUSION: We found three extracts isolated from Chinese medicinal herbs have potential inhibitory activity of Cdc25 phosphatase using a highly specific mechanism-based screen assay for antimitotic drug discovery.
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Dates et versions

hal-00018682 , version 1 (07-02-2006)

Identifiants

  • HAL Id : hal-00018682 , version 1

Citer

H. Yang, S. Zheng, L. Meijer, Li S. M., S. Leclerc, et al.. Screening the active constituents of Chinese medicinal herbs as potent inhibitors of Cdc25 tyrosine phosphatase, an activator of the mitosis-inducing p34cdc2 kinase. Journal of Zhejiang University. Science, 2005, 6, pp.656-63. ⟨hal-00018682⟩
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