Homeostatic regulation of supercoiling sensitivity coordinates transcription of the bacterial genome. - Université Pierre et Marie Curie Accéder directement au contenu
Article Dans Une Revue EMBO Reports Année : 2006

Homeostatic regulation of supercoiling sensitivity coordinates transcription of the bacterial genome.

Résumé

Regulation of cellular growth implies spatiotemporally coordinated programmes of gene transcription. A central question, therefore, is how global transcription is coordinated in the genome. The growth of the unicellular organism Escherichia coli is associated with changes in both the global superhelicity modulated by cellular topoisomerase activity and the relative proportions of the abundant DNA-architectural chromatin proteins. Using a DNA-microarray-based approach that combines mutations in the genes of two important chromatin proteins with induced changes of DNA superhelicity, we demonstrate that genomic transcription is tightly associated with the spatial distribution of supercoiling sensitivity, which in turn depends on chromatin proteins. We further demonstrate that essential metabolic pathways involved in the maintenance of growth respond distinctly to changes of superhelicity. We infer that a homeostatic mechanism organizing the supercoiling sensitivity is coordinating the growth-phase-dependent transcription of the genome.

Dates et versions

hal-00529439 , version 1 (25-10-2010)

Identifiants

Citer

Nicolas Blot, Ramesh Mavathur, Marcel Geertz, Andrew Travers, Georgi Muskhelishvili. Homeostatic regulation of supercoiling sensitivity coordinates transcription of the bacterial genome.. EMBO Reports, 2006, 7 (7), pp.710-5. ⟨10.1038/sj.embor.7400729⟩. ⟨hal-00529439⟩
457 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More