The effect of re-dissolution solvents and HPLC columns on the analysis of mycosporine-like amino acids in the eulittoral macroalgae and

Ulf Karsten (1) , Karine Escoubeyrou (2) , François Charles (2)

1 Institute of Biological Sciences
2 LOBB - Laboratoire d'océanographie biologique de Banyuls

Many macroalgal species that are regularly exposed to high solar radiation such as the eulittoral green alga and the red alga synthesize and accumulate high concentrations of mycosporine-like amino acids (MAAs) as UV-sunscreen compounds. These substances are typically extracted with a widely used standard protocol following quantification by various high performance liquid chromatography (HPLC) techniques. However, further preparation steps prior to HPLC analysis as well as different HPLC column types have not been systematically checked regarding separation quality and reproducibility. Therefore pure methanol, distilled water and HPLC eluent were evaluated as re-dissolution solvent for dried and extracts, which were subsequently analyzed on three reversed-phase C8 and C18 HPLC columns. The data indicate that distilled water and the HPLC eluent gave almost identical peak patterns and MAA contents on the C8 and C18 columns. In contrast, the application of the widely used methanol led to double peaks or even the loss of specific peaks as well as to a strong decline in total MAA amounts ranging from about 35% of the maximum in to 80% of the maximum in . Consequently, methanol should be avoided as re-dissolution solvent for the HPLC sample preparation. An improved protocol for the MAA analysis in macroalgae in combination with a reliable C18 column is suggested.

Mots clés: Photoprotection; Reversed-phase column; UV-sunscreen

Ulf Karsten, Karine Escoubeyrou, François Charles. The effect of re-dissolution solvents and HPLC columns on the analysis of mycosporine-like amino acids in the eulittoral macroalgae and. Helgoland Marine Research, 2009, 63 (3), pp.231-238. ⟨10.1007/s10152-009-0152-0⟩. ⟨hal-00535190⟩