FRET multiphoton spectral imaging microscopy of 7-ketocholesterol and Nile Red in U937 monocytic cells loaded with 7-ketocholesterol.

Edmond Kahn (1) , Anne Vejux (2, 3) , Dominique Dumas (4, 5) , Thomas Montange , Frédérique Frouin (1) , Vincent Robert (6) , Jean-Marc Riedinger (7) , Jean-François Stoltz (4, 5) , Philippe Gambert (8) , Andrew Todd-Pokropek , Gérard Lizard (3)

1 LIF - Laboratoire d'Imagerie Fonctionnelle
2 LBMC - Laboratoire de Biochimie Moléculaire et Cellulaire
3 LNC - Lipides - Nutrition - Cancer (U866)
4 LEMTA - Laboratoire d'Energétique et Mécanique Théorique et Appliquée
5 PTIBC-IBISA Nancy - Plate-forme Imagerie et Biophysique Cellulaire et Tissulaire
6 PAROLE - Analysis, perception and recognition of speech
7 Laboratoire de Biologie Médicale [Centre Georges-François leclerc]
8 LAP - Plateforme Lipidomique [Dijon]

This investigation established the utility of 2-photon excitation CLSM to assess colocalization of 7KC with NR by FRET and to identify and distinguish polar and neutral lipids stained by NR that accumulate from the effect of 7KC.

Domaines: Imagerie

Edmond Kahn, Anne Vejux, Dominique Dumas, Thomas Montange, Frédérique Frouin, et al.. FRET multiphoton spectral imaging microscopy of 7-ketocholesterol and Nile Red in U937 monocytic cells loaded with 7-ketocholesterol.. Analytical and Quantitative Cytology and Histology, 2004, 26 (6), pp.304-13. ⟨hal-00559065⟩