Study of the DNA hybridization transduction behavior of a quinone-containing electroactive polymer by cyclic voltammetry and electrochemical impedance spectroscopy

B. Piro (1) , J. Haccoun (1) , M.C. Pham (1) , L.D. Tran (1) , Anne Rubin (2, 1, 3) , Hubert Perrot (3) , Claude Gabrielli (3)

1 ITODYS (UMR_7086) - Interfaces, Traitements, Organisation et Dynamique des Systèmes
2 ICS - Institut Charles Sadron
3 LISE - Laboratoire Interfaces et Systèmes Electrochimiques

We report the characterization of a new bifunctional electroactive polymer, poly(5-hydroxy-1,4-naphthoquinone (juglone)-co-5-hydroxy-3-thioacetic acid-1,4-naphthoquinone), which can be used for direct electrochemical detection of DNA hybridization. The polymer structure was characterized based on molecular orbital calculations and ex situ and in situ FT-IR spectroscopy. The quinone group integrated in the polymer presents very stable electroactivity in neutral aqueous medium and can act as a redox marker for hybridization. The carboxylic function allows the preparation of oligonucleotides (ODN) probe-modified films by covalent binding. The mechanism of hybridization detection was studied by cyclic voltammetry and electrochemical impedance spectroscopy. The "signal-on" upon hybridization could be attributed to a change in conformation from random ODN coils on the probe-modified film to helix double stranded ODNs upon hybridization.

Mots clés: Oligonucleotide; Electrochemical impedance spectroscopy; Conducting polymer; Cyclic voltammetry; DNA sensor; Quinone

Domaines: Chimie-Physique [physics.chem-ph]; Polymères

B. Piro, J. Haccoun, M.C. Pham, L.D. Tran, Anne Rubin, et al.. Study of the DNA hybridization transduction behavior of a quinone-containing electroactive polymer by cyclic voltammetry and electrochemical impedance spectroscopy. Journal of electroanalytical chemistry and interfacial electrochemistry, 2005, 577 (1), pp.155-165. ⟨10.1016/j.jelechem.2004.12.002⟩. ⟨hal-00630142⟩