Optimal assay conditions for aspartate transcarbamylase (ATCase) activity in mesozooplankton - Université Pierre et Marie Curie Accéder directement au contenu
Article Dans Une Revue Journal of Plankton Research Année : 1998

Optimal assay conditions for aspartate transcarbamylase (ATCase) activity in mesozooplankton

Résumé

The optimal conditions for storing and assaying mesozooplankton aspartate transcarbamylase (ATCase) (EC 2.1.3.1.) are defined in order to enable the specific activity of this enzyme to be used as an index of mesozooplankton productivity. ATCase activity was found to be stable for more than 2 weeks when stored in liquid nitrogen, with a negligible loss of activity. whatever the state of frozen material (cell-free homogenate or whole cells). As an alternative, -20 degrees C,-90 degrees C freezers and dry ice (-80 degrees C) can give more flexibility for storing and transporting material, provided initial freezing has been carried out at -196 degrees C. ATCase specific activity was stable over a large range of total protein concentrations (2-55 mg ml(-1)) for most mesozooplankton samples, and the enzyme activity was linear between 0.8 and 80 nmol carbamyl aspartate min(-1) mg(-1) of total protein. The optimal incubation time (30 min), temperature (35 degrees C) and pH range (9-9.5) for assay of ATCase were similar for Calanus helgolandicus (Crustacea: Copepoda) (G.O.Sars) and three mesozooplankton assemblages sampled off Plymouth (UK) in May, August and November. However, the K-m values for both substrates were variable: from 3.9 to 79.1 for aspartate, and from 0.83 to 3.46 for carbamyl phosphate. Such variations are likely to be due to the presence of different ATCase catalytic potential, rather I:han changes in ATCase assemblage.

Dates et versions

hal-00726590 , version 1 (30-08-2012)

Identifiants

Citer

I.C. Biegal, J.P. Bergeron. Optimal assay conditions for aspartate transcarbamylase (ATCase) activity in mesozooplankton. Journal of Plankton Research, 1998, 20 (7), pp.1205-1218. ⟨10.1093/plankt/20.7.1205⟩. ⟨hal-00726590⟩
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