Ozone and aging up-regulate type II metacaspase gene expression and global metacaspase activity in the leaves of field-grown maize (Zea mays L.) plants - Archive ouverte HAL Access content directly
Journal Articles Chemosphere Year : 2012

Ozone and aging up-regulate type II metacaspase gene expression and global metacaspase activity in the leaves of field-grown maize (Zea mays L.) plants

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Rafiq Ahmad
  • Function : Author
Yasmine Zuily-Fodil
  • Function : Author
Chantal Passaquet
  • Function : Author
Anne Repellin
  • Function : Author

Abstract

Maize plants (Zea mays L. cv. NK Perform) were exposed to O3-enriched air, using a new field fumigation system. Transcriptional changes for three type II-metacaspase genes were studied in the leaves (ranks 10 and 12), using quantitative real-time PCR. Global metacaspase activity was measured using metacaspasespecific synthetic tripeptide Boc-GRR-AMC. Aging had little effect on mRNA accumulation whereas four to six-fold increases were observed for the most O3-responsive type II metacaspase genes, in the older leaves 10. Global metacaspase activity increased by 257% and 333% in leaves 12 and 10, respectively, in response to the highest cumulated concentration. In non-fumigated plants, metacaspase activity progressively increased over the course of the experiment and always was higher in the older leaves 10. Together, these results suggest that metacaspase-mediated proteolysis is a crucial step in leaf responses to both O3 and age-mediated senescence.
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hal-01003248 , version 1 (10-06-2014)

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Rafiq Ahmad, Yasmine Zuily-Fodil, Chantal Passaquet, Olivier Bethenod, Romain Roche, et al.. Ozone and aging up-regulate type II metacaspase gene expression and global metacaspase activity in the leaves of field-grown maize (Zea mays L.) plants. Chemosphere, 2012, 87 (7), pp.789-795. ⟨10.1016/j.chemosphere.2011.12.081⟩. ⟨hal-01003248⟩
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