An eIF4E-interacting peptide induces cell death in cancer cell lines

M Masse (1, 2) , V Glippa (1, 2) , H Saad (3, 1, 2) , R Le Bloas (2, 3) , I Gauffeny (1, 2) , C Berthou (2, 3) , M Czjzek (1, 2) , P Cormier (1, 2) , B Cosson (1, 2)

1 LBI2M - Laboratoire de Biologie Intégrative des Modèles Marins
2 UEB - Université européenne de Bretagne - European University of Brittany
3 Department of Clinical Hematology, Institute of Cancerology and Hematology

The eukaryotic initiation factor eIF4E is essential for cap-dependent initiation of translation in eukaryotes. Abnormal regulation of eIF4E has been implicated in oncogenic transformation. We developed an eIF4E-binding peptide derived from Angel1, a partner of eIF4E that we recently identified. We show here that this peptide fused to a penetratin motif causes drastic and rapid cell death in several epithelial cancer cell lines. This necrotic cell death was characterized by a drop in ATP levels with F-actin network injury being a key step in extensive plasma membrane blebbing and membrane permeabilization. This synthetic eIF4E-binding peptide provides a candidate pharmacophore for a promising new cancer therapy strategy.

Domaines: Biologie cellulaire

M Masse, V Glippa, H Saad, R Le Bloas, I Gauffeny, et al.. An eIF4E-interacting peptide induces cell death in cancer cell lines. Cell Death and Disease, 2014, 5, pp.e1500. ⟨10.1038/cddis.2014.457⟩. ⟨hal-01084159⟩