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Article Dans Une Revue Neuron Année : 2014

Signaling Switch of the Axon Guidance Receptor Robo3 during Vertebrate Evolution

Résumé

Development of neuronal circuits is controlled by evolutionarily conserved axon guidance molecules, including Slits, the repulsive ligands for roundabout (Robo) receptors, and Netrin-1, which mediates attraction through the DCC receptor. We discovered that the Robo3 receptor fundamentally changed its mechanism of action during mammalian evolution. Unlike other Robo receptors, mammalian Robo3 is not a high-affinity receptor for Slits because of specific substitutions in the first immunoglobulin domain. Instead, Netrin-1 selectively triggers phosphorylation of mammalian Robo3 via Src kinases. Robo3 does not bind Netrin-1 directly but interacts with DCC. Netrin-1 fails to attract pontine neurons lacking Robo3, and attraction can be restored in Robo3(-/-) mice by expression of mammalian, but not nonmammalian, Robo3. We propose that Robo3 evolution was key to sculpting the mammalian brain by converting a receptor for Slit repulsion into one that both silences Slit repulsion and potentiates Netrin attraction.
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Dates et versions

hal-01102724 , version 1 (13-01-2015)

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Pavol Zelina, Heike Blockus, Yvrick Zagar, Amélie Péres, François Friocourt, et al.. Signaling Switch of the Axon Guidance Receptor Robo3 during Vertebrate Evolution. Neuron, 2014, 84 (6), pp.1258-1272. ⟨10.1016/j.neuron.2014.11.004⟩. ⟨hal-01102724⟩
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