Genome-wide mosaicism within Mycobacterium abscessus: evolutionary and epidemiological implications - Université Pierre et Marie Curie Accéder directement au contenu
Article Dans Une Revue BMC Genomics Année : 2016

Genome-wide mosaicism within Mycobacterium abscessus: evolutionary and epidemiological implications

Lise Frézal
Roland Brosch
Jean-Louis Herrmann

Résumé

Background: In mycobacteria, conjugation differs from the canonical Hfr model, but is still poorly understood. Here, we quantified this evolutionary processe in a natural mycobacterial population, taking advantage of a large clinical strain collection of the emerging pathogen Mycobacterium abscessus (MAB). Results Multilocus sequence typing confirmed the existence of three M. abscessus subspecies, and unravelled extensive allelic exchange between them. Furthermore, an asymmetrical gene flow occurring between these main lineages was detected, resulting in highly admixed strains. Intriguingly, these mosaic strains were significantly associated with cystic fibrosis patients with lung infections or chronic colonization. Genome sequencing of those hybrid strains confirmed that half of their genomic content was remodelled in large genomic blocks, leading to original tri-modal ‘patchwork’ architecture. One of these hybrid strains acquired a locus conferring inducible macrolide resistance, and a large genomic insertion from a slowly growing pathogenic mycobacteria, suggesting an adaptive gene transfer. This atypical genomic architecture of the highly recombinogenic strains is consistent with the distributive conjugal transfer (DCT) observed in M. smegmatis. Intriguingly, no known DCT function was found in M. abscessus chromosome, however, a p-RAW-like genetic element was detected in one of the highly admixed strains. Conclusion Taken together, our results strongly suggest that MAB evolution is sporadically punctuated by dramatic genome wide remodelling events. These findings might have far reaching epidemiological consequences for emerging mycobacterial pathogens survey in the context of increasing numbers of rapidly growing mycobacteria and M. tuberculosis co-infections.

Domaines

Génétique
Fichier principal
Vignette du fichier
art_10.1186_s12864-016-2448-1.pdf (2.27 Mo) Télécharger le fichier
Origine : Publication financée par une institution
Loading...

Dates et versions

hal-01284527 , version 1 (07-03-2016)

Licence

Paternité

Identifiants

Citer

Guillaume Sapriel, Julie Konjek, Mickael Orgeur, Laurent Bouri, Lise Frézal, et al.. Genome-wide mosaicism within Mycobacterium abscessus: evolutionary and epidemiological implications. BMC Genomics, 2016, 17 (1), pp.118. ⟨10.1186/s12864-016-2448-1⟩. ⟨hal-01284527⟩
794 Consultations
241 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More