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Article Dans Une Revue Research in Microbiology Année : 2017

Utilization of interferometric light microscopy for the rapid analysis of virus abundance in a river

Résumé

There is a constant need for direct counting of biotic nanoparticles such as viruses to unravel river functioning. We used, for the first time in freshwater, a new method based on interferometry differentiating viruses from other particles such as membrane vesicles. In the French Marne River, viruses represented between 42 and 72% of the particles. A spring monitoring in 2014 revealed their increase (2.1 × 107 to 2.1 × 108 mL−1) linked to an increase in algal biomass and diversity of bacterial plankton. Predicted virus size distributions were in agreement with transmission electron microscopy analysis suggesting a dominance of large viruses (≥60 nm).
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Dates et versions

hal-01497684 , version 1 (29-03-2017)

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Céline Roose-Amsaleg, Yasmina Fedala, Catherine Vénien-Bryan, Josette Garnier, Albert-Claude Boccara, et al.. Utilization of interferometric light microscopy for the rapid analysis of virus abundance in a river. Research in Microbiology, 2017, ⟨10.1016/j.resmic.2017.02.004⟩. ⟨hal-01497684⟩
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