Naturally occurring steroids in Xenopus oocyte during meiotic maturation. Unexpected presence and role of steroid sulfates - Archive ouverte HAL Access content directly
Journal Articles Molecular and Cellular Endocrinology Year : 2012

Naturally occurring steroids in Xenopus oocyte during meiotic maturation. Unexpected presence and role of steroid sulfates

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1
Olivier Haccard
Philippe Liere
  • Function : Author
Antoine Pianos
  • Function : Author
Bernard Eychenne
  • Function : Author
Catherine Jessus
  • Function : Author
Rene Ozon
  • Function : Author

Abstract

In the ovary, oocytes are surrounded by follicle cells and arrested in prophase of meiosis I. Although steroidogenic activity of follicle cells is involved in oogenesis regulation, clear qualitative and quantitative data about the steroid content of follicles are missing. We measured steroid levels of Xenopus oocytes and follicles by gas chromatography-mass spectrometry. We show that dehydroepiandrosterone sulfate is the main steroid present in oocytes. Lower levels of free steroids are also detected, e.g., androgens, whereas progesterone is almost undetectable. We propose that sulfatation is a protective mechanism against local variations of active steroids that could be deleterious for follicle-enclosed oocytes. Steroid levels were measured after LH stimulation, responsible for the release by follicle cells of a steroid signal triggering oocyte meiosis resumption. Oocyte levels of androgens rise slowly during meiosis re-entry whereas progesterone increases abruptly to micromolar concentration, therefore representing the main physiological mediator of meiosis resumption in Xeno pus oocyte.
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Dates and versions

hal-01538514 , version 1 (13-06-2017)

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Olivier Haccard, Aude Dupre, Philippe Liere, Antoine Pianos, Bernard Eychenne, et al.. Naturally occurring steroids in Xenopus oocyte during meiotic maturation. Unexpected presence and role of steroid sulfates. Molecular and Cellular Endocrinology, 2012, 362 (1-2), pp.110-119. ⟨10.1016/j.mce.2012.05.019⟩. ⟨hal-01538514⟩
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