Non-centrosomal epidermal microtubules act in parallel to LET-502/ROCK to promote C. elegans elongation - Archive ouverte HAL Access content directly
Journal Articles Development (Cambridge, England) Year : 2016

Non-centrosomal epidermal microtubules act in parallel to LET-502/ROCK to promote C. elegans elongation

, , , , , , , , (1) ,
1
Shahoe Wang
  • Function : Author
Julien Pontabry
  • Function : Author
Ambre Bender
  • Function : Author
Francois Robin
  • Function : Author
Vincent Hyenne
  • Function : Author
Frederic Landmann
Christelle Gally
  • Function : Author
Karen Oegema
  • Function : Author
Sophie Quintin
  • Function : Author

Abstract

C. elegans embryonic elongation is a morphogenetic event driven by actomyosin contractility and muscle-induced tension transmitted through hemidesmosomes. A role for the microtubule cytoskeleton has also been proposed, but its contribution remains poorly characterized. Here, we investigate the organization of the non-centrosomal microtubule arrays present in the epidermis and assess their function in elongation. We show that the microtubule regulators.-tubulin and NOCA-1 are recruited to hemidesmosomes and adherens junctions early in elongation. Several parallel approaches suggest that microtubule nucleation occurs from these sites. Disrupting the epidermal microtubule array by overexpressing the microtubule-severing protein Spastin or by inhibiting the C. elegans ninein homolog NOCA-1 in the epidermis mildly affected elongation. However, microtubules were essential for elongation when hemidesmosomes or the activity of the Rho kinase LET-502/ROCK were partially compromised. Imaging of junctional components and genetic analyses suggest that epidermal microtubules function together with Rho kinase to promote the transport of E-cadherin to adherens junctions and myotactin to hemidesmosomes. Our results indicate that the role of LET-502 in junctional remodeling is likely to be independent of its established function as a myosin II activator, but requires a microtubule-dependent pathway involving the syntaxin SYX-5. Hence, we propose that non-centrosomal microtubules organized by epidermal junctions contribute to elongation by transporting junction remodeling factors, rather than having a mechanical role.

Dates and versions

hal-01538537 , version 1 (13-06-2017)

Identifiers

Cite

Shahoe Wang, Julien Pontabry, Ambre Bender, Francois Robin, Vincent Hyenne, et al.. Non-centrosomal epidermal microtubules act in parallel to LET-502/ROCK to promote C. elegans elongation. Development (Cambridge, England), 2016, 143 (1), pp.160-173. ⟨10.1242/dev.126615⟩. ⟨hal-01538537⟩
31 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More