Relationships between Cortical Bone Quality Biomarkers: Stiffness, Toughness, Microstructure, Mineralization, Cross-Links and Collagen - Université Pierre et Marie Curie Accéder directement au contenu
Communication Dans Un Congrès Année : 2017

Relationships between Cortical Bone Quality Biomarkers: Stiffness, Toughness, Microstructure, Mineralization, Cross-Links and Collagen

Quentin Grimal

Résumé

Bone quality encompasses bone properties that contribute to fracture risk, such as bone stiffness, microstructure, matrix constituents or tissue material properties. These aspects cannot be quantified in-vivo except for stiffness, a surrogate biomarker of strength, which can be assessed using quantitative ultrasound techniques. To better predict bone fracturerisk, investigating the relationships between stiffness and other bone quality factors is important. Toward this goal, our group adapted resonant ultrasound spectroscopy (RUS) to precisely measure the whole set of stiffness coefficients of cortical bone by improving the signal processing and automatizing the inversion procedure based on a Bayesian framework. In this work, we present the relationships between bone quality biomarkers including stiffness, fracture toughness, microstructure, mineralization, cross-links and collagen.Statement of Contribution/MethodsFrom the mid-diaphysis of the femoral cortex of 27 human donors, 54 cuboid specimens (3x4x5 mm3, axis 1=radial; axis 2=circumferential; axis 3=axial) were prepared. The mesoscale transverse isotropic stiffness tensor Cij of the specimens was measured by RUS. The microstructure (i.e., vascular porosity) and degree of mineralization of bone (DMB)were estimated from synchrotron radiation micro-computed tomography (voxel size 6.5 ?m). Another set of 54 samples (3x4x25 mm3) which were adjacent to the previous ones, was harvested for mechanical testing. Each of them were divided into two samples (1x2x25 mm3) then notched (1 mm length) in their middle in the transverse direction. Three-pointbending tests were conducted at two strain rates 10-4 s-1 and 10-1 s-1 to measure the fracture toughness (quasi-static KJcStat and dynamic KjcDyn). Biochemical tests were conducted on small adjacent bone fragments to assess collagen quantity and cross-links.Results/DiscussionWhile all the stiffness coefficients significantly correlate with toughness (-0.35 < r < -0.53, p < 0.05) except for C33, different biomarkers seem to differently affect toughness and stiffness. Indeed, markers that showed an effect on toughness did not on stiffness (Table 1). This work demonstrates the correlations between cortical bone quality biomarkers and givesinsights for a better assessment of bone fracture risk using stiffness measured in-vivo.
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Dates et versions

hal-01598039 , version 1 (29-09-2017)

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  • HAL Id : hal-01598039 , version 1

Citer

Xiran Cai, Rémy Gauthier, Laura Peralta, Hélène Follet, Evelyne Gineyts, et al.. Relationships between Cortical Bone Quality Biomarkers: Stiffness, Toughness, Microstructure, Mineralization, Cross-Links and Collagen. Ultrasonics Symposium (IUS), 2017 IEEE International, Sep 2017, WASHINGTON, United States. ⟨hal-01598039⟩
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