Determination of chlorophylls and carotenoids of marine phytoplankton: Separation of chlorophyll a from divinyl-chlorophyll a and zeaxanthin from lutein
Résumé
A rapid reverse-phase HPLC method is presented for the identification and quantification of most of the phytoplankton pigments. This method yields the resolution of divinyl-chlorophyll a and chlorophyll a, as well as the partial resolution of lutein and zeaxanthin, and of divinyl-chlorophyll b and chlorophyll b. In addition, chlorophylls c(1,2) and c(3) are well resolved. The analysis time for one sample is 20 min, which makes this method particularly suited when large numbers of samples have to be processed.