Enrichment of antigen-specific T lymphocytes by panning on immobilized MHC-peptide complexes.

Philippe Bousso (1) , Frédérique Michel (2) , Nathalie Pardigon (1) , Nadege Bercovici (3) , Roland Liblau (3) , Philippe Kourilsky (1) , Jean-Pierre Abastado (1)

1 Biologie Moléculaire du Gène
2 Immunologie Moléculaire
3 Laboratoire d'Immunologie Cellulaire et Clinique

Numerous studies have focused on characterizing and monitoring antigen-specific T cells during the course of an immune response. Mostly indirect methods were used to circumvent the low frequency of T cell precursors and the inherent complexity of T cell receptor (TcR)-MHC-peptide interactions. Here, we took advantage of peptide-specific adhesion induced by immobilized MHC-peptide complexes. We describe a simple technique which allows enrichment in antigen-specific T lymphocytes among a heterogeneous CD8+ T cell population. Enrichment of T cells according to their specificity should facilitate their characterization and provide an attractive tool for immunotherapy.

Mots clés: T cell activation; MHC; Panning

Domaines: Immunité innée

Philippe Bousso, Frédérique Michel, Nathalie Pardigon, Nadege Bercovici, Roland Liblau, et al.. Enrichment of antigen-specific T lymphocytes by panning on immobilized MHC-peptide complexes.. Immunology Letters, 1997, 59 (2), pp.85-91. ⟨10.1016/S0165-2478(97)00105-3⟩. ⟨pasteur-01659378⟩