αII-βV spectrin bridges the plasma membrane and cortical lattice in the lateral wall of the auditory outer hair cells - Université Pierre et Marie Curie Accéder directement au contenu
Article Dans Une Revue Journal of Cell Science Année : 2008

αII-βV spectrin bridges the plasma membrane and cortical lattice in the lateral wall of the auditory outer hair cells

Résumé

The sensitivity and frequency selectivity of the mammalian cochlea involves a mechanical amplification process called electromotility, which requires prestin-dependent length changes of the outer hair cell (OHC) lateral wall in response to changes in membrane electric potential. The cortical lattice, the highly organized cytoskeleton underlying the OHC lateral plasma membrane, is made up of F-actin and spectrin. Here, we show that αII and two of the five β-spectrin subunits, βII and βV, are present in OHCs. βII spectrin is restricted to the cuticular plate, a dense apical network of actin filaments, whereas βV spectrin is concentrated at the cortical lattice. Moreover, we show that αII-βV spectrin directly interacts with F-actin and band 4.1, two components of the OHC cortical lattice. βV spectrin is progressively recruited into the cortical lattice between postnatal day 2 (P2) and P10 in the mouse, in parallel with prestin membrane insertion, which itself parallels the maturation of cell electromotility. Although βV spectrin does not directly interact with prestin, we found that addition of lysates derived from mature auditory organs, but not from the brain or liver, enables βV spectrin–prestin interaction. Using this assay, βV spectrin, via its PH domain, indirectly interacts with the C-terminal cytodomain of prestin. We conclude that the cortical network involved in the sound-induced electromotility of OHCs contains αII-βV spectrin, and not the conventional αII-βII spectrin.
Fichier principal
Vignette du fichier
3347.pdf (1.48 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
licence : Copyright (Tous droits réservés)

Dates et versions

pasteur-03926801 , version 1 (06-01-2023)

Licence

Copyright (Tous droits réservés)

Identifiants

Citer

Kirian Legendre, Saaid Safieddine, Polonca Küssel-Andermann, Christine Petit, Aziz El-Amraoui. αII-βV spectrin bridges the plasma membrane and cortical lattice in the lateral wall of the auditory outer hair cells. Journal of Cell Science, 2008, 121 (20), pp.3347-3356. ⟨10.1242/jcs.028134⟩. ⟨pasteur-03926801⟩
15 Consultations
48 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More