Rapid and early determination of sex using trophoblast biopsy specimens and Y chromosome specific DNA probes.

Gilles Vergnaud 1, 2 L Kaplan J Weissenbach 3 Y Dumez R Berger P Tiollais G Guellaen 4
1 IGM - Institut de génétique et microbiologie [Orsay]
2 ENSTA Paris - École Nationale Supérieure de Techniques Avancées
3 GENOSCOPE - Genoscope - Centre national de séquençage [Evry]
4 IMRB - Institut Mondor de Recherche Biomédicale
Abstract : The feasibility of determining sex by analysing deoxyribonucleic acid (DNA) with two probes specific for Y chromosomes was shown using DNA obtained from samples of blood from 30 non-related males and females of different ethnic origin. The DNA was spotted on nitrocellulose filters and hybridised with both a repetitive (P1) and a unique (49f) sequence specific for the human Y chromosome. A strong positive signal with both probes indicated the presence of male DNA. The sex of 12 fetuses was then similarly determined by molecular characterisation of DNA from trophoblast biopsy specimens. Chorionic samples were obtained in seven cases before termination of pregnancy in the first trimester and the aborted embryos subjected to karyotyping and sex chromatin analysis. In the five other cases samples were obtained from placentas obtained during caesarean section. Results of hybridisation were compared with those from cytogenic studies and actual sex at birth. The sex of all 12 fetuses was determined correctly by hybridisation.
Document type :
Journal articles
Complete list of metadatas
https://hal-ensta-paris.archives-ouvertes.fr//hal-01160714
Contributor : Gilles Vergnaud <>
Submitted on : Saturday, June 6, 2015 - 5:58:01 PM
Last modification on : Thursday, October 10, 2019 - 11:36:02 AM

Links full text

pubmedcentral

Identifiers

Collections

ENSTA | IMRB | CEA | UPEC-UPEM | I2BC | JACOB | CEA-DRF | UPEC | GENOSCOPE

Citation

Gilles Vergnaud, L Kaplan, J Weissenbach, Y Dumez, R Berger, et al.. Rapid and early determination of sex using trophoblast biopsy specimens and Y chromosome specific DNA probes.. British medical journal (Clinical research ed.), 1984, 289 (6437), pp.73-6. ⟨hal-01160714⟩

Export

BibTeX TEI DC DCterms EndNote

Share

Metrics

Record views

400

Contact

support.ccsd.cnrs.fr
hal.support@ccsd.cnrs.fr
Logo CCSD