Rapid and early determination of sex using trophoblast biopsy specimens and Y chromosome specific DNA probes. - ENSTA Paris - École nationale supérieure de techniques avancées Paris Accéder directement au contenu
Article Dans Une Revue British medical journal (Clinical research ed.) Année : 1984

Rapid and early determination of sex using trophoblast biopsy specimens and Y chromosome specific DNA probes.

Résumé

The feasibility of determining sex by analysing deoxyribonucleic acid (DNA) with two probes specific for Y chromosomes was shown using DNA obtained from samples of blood from 30 non-related males and females of different ethnic origin. The DNA was spotted on nitrocellulose filters and hybridised with both a repetitive (P1) and a unique (49f) sequence specific for the human Y chromosome. A strong positive signal with both probes indicated the presence of male DNA. The sex of 12 fetuses was then similarly determined by molecular characterisation of DNA from trophoblast biopsy specimens. Chorionic samples were obtained in seven cases before termination of pregnancy in the first trimester and the aborted embryos subjected to karyotyping and sex chromatin analysis. In the five other cases samples were obtained from placentas obtained during caesarean section. Results of hybridisation were compared with those from cytogenic studies and actual sex at birth. The sex of all 12 fetuses was determined correctly by hybridisation.

Dates et versions

hal-01160714 , version 1 (06-06-2015)

Identifiants

Citer

Gilles Vergnaud, L Kaplan, J Weissenbach, Y Dumez, R Berger, et al.. Rapid and early determination of sex using trophoblast biopsy specimens and Y chromosome specific DNA probes.. British medical journal (Clinical research ed.), 1984, 289 (6437), pp.73-6. ⟨10.1136/bmj.289.6437.73⟩. ⟨hal-01160714⟩
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