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Journal Articles eLife Year : 2022

A mechano-osmotic feedback couples cell volume to the rate of cell deformation

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Nishit Srivastava
Matthieu Piel

Abstract

Mechanics has been a central focus of physical biology in the past decade. In comparison, how cells manage their size is less understood. Here, we show that a parameter central to both the physics and the physiology of the cell, its volume, depends on a mechano-osmotic coupling. We found that cells change their volume depending on the rate at which they change shape, when they spontaneously spread or when they are externally deformed. Cells undergo slow deformation at constant volume, while fast deformation leads to volume loss. We propose a mechanosensitive pump and leak model to explain this phenomenon. Our model and experiments suggest that volume modulation depends on the state of the actin cortex and the coupling of ion fluxes to membrane tension. This mechano-osmotic coupling defines a membrane tension homeostasis module constantly at work in cells, causing volume fluctuations associated with fast cell shape changes, with potential consequences on cellular physiology.
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Origin : Publication funded by an institution

Dates and versions

hal-03773604 , version 1 (05-10-2022)

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Larisa Venkova, Amit Singh Vishen, Sergio Lembo, Nishit Srivastava, Baptiste Duchamp, et al.. A mechano-osmotic feedback couples cell volume to the rate of cell deformation. eLife, 2022, 11, ⟨10.7554/eLife.72381⟩. ⟨hal-03773604⟩
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