Development of a set of SNP markers for population genetics of the sea rose, Pentapora fascialis

Mariana Padrón (1) , M. Milhes (2) , M. Massot (3) , Erwan Guichoux (3) , Katell Guizien (1)

1 LECOB - Laboratoire d'Ecogéochimie des environnements benthiques
2 GeT-PlaGe - Génome et Transcriptome - Plateforme Génomique
3 BioGeCo - Biodiversité, Gènes & Communautés

A set of single nucleotide polymorphisms (SNP) was developed from the transcriptome of 10 individuals of the sea rose Pentapora fascialis (Cheilostomata) collected in Banyuls-sur-Mer Bay (NW Mediterranean sea). 53042 putative SNPs were identified and mapped on a de novo assembled transcriptome. A selected set of 320 SNPs with highest coverage and uniquely mapped in the assembled transcriptome was tested using a MassARRAY System on 95 individuals sampled in natural rocks and artificial reefs distributed over a hundred kilometers in the NW Mediterranean (outisde Banyuls-sur-Mer Bay). A total of 177 SNPs were successfully genotyped and found to be polymorphic. Among these, 154 SNPs were in Hardy-Weinberg equilibrium over all samples, with significant linkage disequilibrium in only 21 pairs of SNPs. The newly developed loci will be a valuable tool for population genetics studies of this calcifying bryozoan species whose erect structure makes it an engineering species and a target for conservation plans.

Mots clés: Pentapora fascialis; Single nucleotide polymorphism; MassARRAY; bryozoan

Domaines: Sciences de l'environnement; Biodiversité et Ecologie

Mariana Padrón, M. Milhes, M. Massot, Erwan Guichoux, Katell Guizien. Development of a set of SNP markers for population genetics of the sea rose, Pentapora fascialis. Conservation Genetics Resources, 2024, ⟨10.1007/s12686-024-01348-4⟩. ⟨hal-04501018⟩