Comparative study of three analysis methods (TTGE, flow cytometry and HPLC) for xenobiotic impact assessment on phytoplankton communities - Archive ouverte HAL Access content directly
Journal Articles Ecotoxicology Year : 2009

Comparative study of three analysis methods (TTGE, flow cytometry and HPLC) for xenobiotic impact assessment on phytoplankton communities

(1) , (2) , (3) , (2) , (4) , (2)
1
2
3
4

Abstract

The impacts of the fungicide Opus® (epoxiconazole) on marine phytoplankton communities were assessed in a 12-day field experiment using in situ microcosms maintained underwater at 6 m depth. Three community analysis methods were compared for their sensitivity threshold in fungicide impact detection. When phytoplankton communities were exposed to 1 μg l−1 of epoxiconazole, no effects could be demonstrated using TTGE (Temporal Temperature Gradient gel Electrophoresis), flow cytometry or HPLC. When exposed to 10 μg l−1, TTGE fingerprints from PCR amplified 18S rDNA of communities exhibited significant differences compared with controls (ANOSIM, P = 0.028). Neither flow cytometry counts, nor HPLC pigment profiles allowed to show significant differences in microcosms exposed to 10 μg l−1 of epoxiconazole. When exposed to 100 μg l−1, all three methods allowed to detect significant differences in treated microcosms, as compared to controls. The TTGE analysis appears in this study as the most sensitive method for fungicide impact assessment on eukaryote microbial communities.

Dates and versions

hal-00451105 , version 1 (28-01-2010)

Identifiers

Cite

Sabine Stachowski-Haberkorn, Louis Quiniou, Beatriz Beker, Hansy Haberkorn, Dominique Marie, et al.. Comparative study of three analysis methods (TTGE, flow cytometry and HPLC) for xenobiotic impact assessment on phytoplankton communities. Ecotoxicology, 2009, 18 (3), pp.364-376. ⟨10.1007/s10646-008-0288-8⟩. ⟨hal-00451105⟩
177 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More