Deep sequencing revealed genome-wide single-nucleotide polymorphism and plasmid content of Erwinia amylovora strains isolated in Middle Atlas, Morocco

Najat Hannou (1, 2) , Samuel Mondy (2) , Sara Planamente (2) , Mohieddine Moumni (1) , Pablo Llop (3) , Maria Lopez (3) , Charles Manceau (4) , Marie-Anne Barny (5) , Denis Faure (2)

1 Fac Sci, Dept Biol
2 ISV - Institut des sciences du végétal
3 IVIA - Instituto Valenciano de Investigaciones Agrarias - Institut Valencià d'Investigacions Agraries - Valencian Institute for agricultural Research
4 IRHS - Institut de Recherche en Horticulture et Semences
5 IPP - Interactions Plantes Pathogènes

Erwinia amylovora causes economic losses that affect pear and apple production in Morocco. Here, we report comparative genomics of four Moroccan E. amylovora strains with the European strain CFBP1430 and North-American strain ATCC49946. Analysis of single nucleotide polymorphisms (SNPs) revealed genetic homogeneity of Moroccan's strains and their proximity to the European strain CFBP1430. Moreover, the collected sequences allowed the assembly of a 65 kpb plasmid, which is highly similar to the plasmid pEI70 harbored by several European E. amylovora isolates. This plasmid was found in 33% of the 40 E. amylovora strains collected from several host plants in 2009 and 2010 in Morocco. (C) 2013 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.

Mots clés: Erwinia amylovora; Genome; Plasmid; SNP; Virulence; Fire blight; PATHOGEN; ORGANIZATION; DIVERSITY; PEA29; PCR

Domaines: Sciences du Vivant [q-bio]

Najat Hannou, Samuel Mondy, Sara Planamente, Mohieddine Moumni, Pablo Llop, et al.. Deep sequencing revealed genome-wide single-nucleotide polymorphism and plasmid content of Erwinia amylovora strains isolated in Middle Atlas, Morocco. Research in Microbiology, 2013, 164 (8), pp.815 - 820. ⟨10.1016/j.resmic.2013.06.003⟩. ⟨hal-01209922⟩