Coupling fission and exit of RAB6 vesicles at Golgi hotspots through kinesin-myosin interactions

Stephanie Miserey-Lenkei (1) , Hugo Bousquet (1) , Olena Pylypenko (2) , Sabine Bardin (1) , Ariane Dimitrov (1) , Gaëlle Bressanelli (2) , Raja Bonifay (2) , Vincent Fraisier (3) , Catherine Guillou (4) , Cécile Bougeret (5) , Anne Houdusse (2) , Arnaud Echard (6) , Bruno Goud (1)

1 Mécanismes moléculaires du transport intracellulaire
2 Motilité structurale
3 PICT-IBiSA - BioImaging Cell and Tissue Core Facility
4 ICSN - Institut de Chimie des Substances Naturelles
5 Biokinesis
6 Trafic membranaire et Division cellulaire - Membrane Traffic and Cell Division

The actin and microtubule cytoskeletons play important roles in Golgi structure and function, but how they are connected remain poorly known. In this study, we investigated whether RAB6 GTPase, a Golgi-associated RAB involved in the regulation of several transport steps at the Golgi level, and two of its effectors, Myosin IIA and KIF20A participate in the coupling between actin and microtubule cytoskeleton. We have previously shown that RAB6-Myosin IIA interaction is critical for the fission of RAB6-positive transport carriers from Golgi/TGN membranes. Here we show that KIF20A is also involved in the fission process and serves to anchor RAB6 on Golgi/TGN membranes near microtubule nucleating sites. We provide evidence that the fission events occur at a limited number of hotspots sites. Our results suggest that coupling between actin and microtubule cytoskeletons driven by Myosin II and KIF20A ensures the spatial coordination between RAB6-positive vesicles fission from Golgi/TGN membranes and their exit along microtubules.

Mots clés: Golgi; Membrane fission; Small GTPases; Transport carrier

Domaines: Sciences du Vivant [q-bio]

Stephanie Miserey-Lenkei, Hugo Bousquet, Olena Pylypenko, Sabine Bardin, Ariane Dimitrov, et al.. Coupling fission and exit of RAB6 vesicles at Golgi hotspots through kinesin-myosin interactions. Nature Communications, 2017, 8 (1), pp.1254. ⟨10.1038/s41467-017-01266-0⟩. ⟨hal-02349571⟩